StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.1) Schmidt TGM and Skerra A. The random peptide-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng.1993; 6:109-22. More
StrepII-tag antibody was raised against the amino acid sequence "asWSHPQFEKga".
ELISAWB
Tag
Western blot analysis of (A) 5 and (B) 25ng of StrepII-tagged recombinant protein with StrepII antibody at 1 μg/mL.