AKT1 (phospho Thr450) Antibody
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產(chǎn)品名稱: AKT1 (phospho Thr450) Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
AKT1 (phospho Thr450) Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。AKT1 (phospho Thr450) Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
AKT1 (phospho Thr450) Antibody
的詳細(xì)介紹
AKT1 (phospho Thr450) AntibodyProduct Profile
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Product Name
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AKT1 (phospho Thr450) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.1) Xing J, et al. (1998) Mol Cell Biol 18 (4): 1946-55. Tan Y, et al. (1996) EMBO J; 15 (17): 4629-42. Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385. Mayo LD, et al. (2001) Biol Chem; 276 (27): 25184-9.
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Immunogen
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AKT1 (phospho-Thr450) antibody was raised against a peptide sequence around phosphorylation site of threonine 450 (T-I-T (p) -P-P) derived from Human AKT1.
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AKT1 (phospho Thr450) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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IFIHCWB
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Species Reactivity
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HumanMouseRat
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Concentration
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1mg/ml
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Purification
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Affinity purified
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AKT1 (phospho Thr450) AntibodyTarget Information
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Gene Symbol
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AKT1
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Alternative Names
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AKT
PKB
RAC
CWS6
PRKBA
PKB-ALPHA
RAC-ALPHA
CTNNB
OK/SW-cl.35
PRO2286
RAC-PK-alpha
Protein kinase B
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Molecular Weight(MW)
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60 kDa
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Tissue Specificity
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This antibody detects endogenous level of AKT1 only when phosphorylated at threonine 450.
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AKT1 (phospho Thr450) AntibodyDatabase Links
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Entrez Gene
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207
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Protein Accession
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NP_001014431.1
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AKT1 (phospho Thr450) AntibodyApplication
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Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using AKT1 (phospho-Thr450).
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Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 (Phospho-Thr450). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using AKT1 (phospho-Thr450).
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Application Notes
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store antibody at -20?C for up to one year.
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Storage Buffer
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Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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