Rb (phospho Ser795) Antibody
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產(chǎn)品名稱: Rb (phospho Ser795) Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
Rb (phospho Ser795) Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。Rb (phospho Ser795) Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
Rb (phospho Ser795) Antibody
的詳細(xì)介紹
Rb (phospho Ser795) AntibodyProduct Profile
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Product Name
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Rb (phospho Ser795) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.1) Fang Sun, Hanjiang Fu, Qin Liu, et al. (2008) Downregulation of CCND1 and CDK6 by miR-34a induces cell cyclearrest. FEBS Letters, 582:1564–1568.
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Immunogen
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Rb (Phospho-Ser795) antibody was raised against a peptide sequence around phosphorylation site of serine 795 (P-S-S (p) -P-L) derived from Human Rb.
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Rb (phospho Ser795) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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IFIHCWB
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Species Reactivity
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HumanMouseRat
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Concentration
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1mg/ml
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Purification
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Affinity purified
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Rb (phospho Ser795) AntibodyTarget Information
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Gene Symbol
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RB1
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Alternative Names
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RB
pRb
OSRC
pp110
p105-Rb
ASK1
MAPKKK5
MEKK5
P105-RB
PP105
PP110
RB-1
RB1
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Molecular Weight(MW)
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110 kDa
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Tissue Specificity
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This antibody detects endogenous level of Rb only when phosphorylated at serine 795.
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Rb (phospho Ser795) AntibodyDatabase Links
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Entrez Gene
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5925
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Protein Accession
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NP_000312.2
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Rb (phospho Ser795) AntibodyApplication
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Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb (Phospho-Ser795). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Rb (Phospho-Ser795).
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Application
Western blot analysis of lysed extracts from K562 cells untreated (Lane 1) or treated with serum (lane 2) using Rb (Phospho-Ser795).
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Application Notes
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store antibody at -20?C for up to one year.
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Storage Buffer
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Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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