Akt (phospho Ser473) Antibody
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產(chǎn)品名稱: Akt (phospho Ser473) Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
Akt (phospho Ser473) Antibody迅速增長(zhǎng)的產(chǎn)品線包括一抗、二抗、**測(cè)定及細(xì)胞檢測(cè)試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。Akt (phospho Ser473) Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
Akt (phospho Ser473) Antibody
的詳細(xì)介紹
Akt (phospho Ser473) AntibodyProduct Profile
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Product Name
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Akt (phospho Ser473) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.1) Baudhuin LM, et al. (2004) FASEB J Feb; 18 (2): 341-3. Min YH, et al. (2004) Cancer Res; 64 (15): 5225-31. Feng J, et al. (2004) J Biol Chem; 279 (34): 35510-7.
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Immunogen
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Akt (Phospho-Ser473) antibody was raised against a peptide sequence around phosphorylation site of serine 473 (Q-F-S (p) -Y-S) derived from Human Akt.
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Akt (phospho Ser473) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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IHCWB
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
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Species Reactivity
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HumanMouseRat
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Concentration
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1mg/ml
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Purification
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Affinity purified
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Akt (phospho Ser473) AntibodyTarget Information
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Gene Symbol
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AKT1
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Alternative Names
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AKT
PKB
RAC
CWS6
PRKBA
PKB-ALPHA
RAC-ALPHA
NFKB3
C-AKT
PKB-alpha
RAC-PK-alpha
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Molecular Weight(MW)
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60 kDa
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Tissue Specificity
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This antibody detects endogenous level of Akt only when phosphorylated at serine 473.
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Akt (phospho Ser473) AntibodyDatabase Links
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Entrez Gene
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207
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Protein Accession
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NP_001014431.1
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Akt (phospho Ser473) AntibodyApplication
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Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF, Serum or both using Akt (Phospho-Ser473).
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Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Phospho-Ser473).
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Application Notes
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store antibody at -20?C for up to one year.
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Storage Buffer
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Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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