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產(chǎn)品資料

PTK2 Antibody

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產(chǎn)品名稱: PTK2 Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

PTK2 Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。PTK2 Antibody嚴(yán)格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。


PTK2 Antibody  的詳細(xì)介紹

PTK2 AntibodyProduct Profile

Product Name PTK2 Antibody
Antibody Type Primary Antibodies
Product description 
					

						PTK2 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.1) Calalb,M.B., et.al., Mol. Cell. Biol. 15 (2), 954-963 (1995)
					

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Immunogen 
This PTK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-423 amino acids from the Central region of human PTK2.

PTK2 AntibodyKey Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

FACSIFIHC-PWB

For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:
Species Reactivity

Human

Concentration 1mg/ml
Purification Unpurified

PTK2 AntibodyTarget Information

Gene Symbol PTK2
Alternative Names
Focal adhesion kinase 1
FADK 1
Focal adhesion kinase-related nonkinase
FRNK
Protein phosphatase 1 regulatory subunit 71
PPP1R71
Protein-tyrosine kinase 2
p125FAK
pp125FAK
PTK2
FAK
FAK1
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Molecular Weight(MW) 119 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Rat

PTK2 AntibodyDatabase Links

Entrez Gene 5747
Protein Accession Q05397

PTK2 AntibodyApplication

  • Application

    Confocal immunofluorescent analysis of PTK2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

  • Application

    Formalin-fixed and paraffin-embedded human brain tissue reacted with PTK2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Application

    Flow cytometric analysis of K562 cells using PTK2 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Application

    Western blot analysis of PTK2 Antibody in T47D cell line lysates (35ug/lane)

  • Application

    Western blot analysis of PTK2 using rabbit polyclonal PTK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK2 gene (Lane 2).

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

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