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產(chǎn)品資料

RB1 Antibody

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產(chǎn)品名稱: RB1 Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔

簡(jiǎn)單介紹

RB1 Antibody迅速增長(zhǎng)的產(chǎn)品線包括一抗、二抗、**測(cè)定及細(xì)胞檢測(cè)試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。RB1 Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。


RB1 Antibody  的詳細(xì)介紹

RB1 AntibodyProduct Profile

Product Name RB1 Antibody
Antibody Type Primary Antibodies
Product description 
					

						RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. This protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1.1) Connell-Crowley,L., et.al., Mol. Biol. Cell(2), 287-301 (1997)
					

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Immunogen 
This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 858-886 amino acids from the C-terminal region of human RB1.

RB1 AntibodyKey Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

FACSIHC-PWB

For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:
Species Reactivity

Human

Concentration 1mg/ml
Purification Unpurified

RB1 AntibodyTarget Information

Gene Symbol RB1
Alternative Names
Retinoblastoma-associated protein
p105-Rb
pRb
Rb
pp110
RB1
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Molecular Weight(MW) 106 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Rat

RB1 AntibodyDatabase Links

Entrez Gene 5925
Protein Accession P06400

RB1 AntibodyApplication

  • Application

    Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Application

    Western blot analysis of RB1 Antibody in 293 cell line lysates (35ug/lane)(2ug/ml)

  • Application

    Formalin-fixed and paraffin-embedded human testis tissue reacted with RB1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Application

    Western Blot at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

Application Notes
For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50:

Additional Information

Form Liquid
Storage Instructions Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

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