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產(chǎn)品名稱: p70 S6 Kinase Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
p70 S6 Kinase Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。p70 S6 Kinase Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
p70 S6 Kinase Antibody
的詳細(xì)介紹
p70 S6 Kinase AntibodyProduct Profile
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Product Name
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p70 S6 Kinase Antibody
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Antibody Type
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Primary Antibodies
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Product description
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RPS6KB1 phosphorylates the Ribosomal Protein-S6. Activation of RPS6KB1 requires a complex, ordered series of conformational changes and phosphorylation reactions. While the role of sequential, multi-site phosphorylation has been extensively detailed, characterization of the priming step required to initiate this cascade has remained elusive. Probably this priming process is dependent on calcium. Calcium-dependent regulation of RPS6KB1 does not specifically target Thr-229 and Thr-389, the key regulatory phosphorylation sites; rather, calcium chelation results in a global inhibition of RPS6KB1 phosphorylation. The initial calcium-dependent process is required to release an inhibitory interaction between the C- and N-termini of RPS6KB1, thus allowing phosphorylation of key domains. The priming event involves formation of a calcium-dependent protein complex that releases the interaction between the N- and C-termini. RPS6KB1 is then accessible for activation by the kinases that target the known regulatory phosphorylation sites .1) Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851Papst PJ, et al. (1998) J Biol Chem. 273 (24): 15077-84. Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 cLe, X. F, et al. (2003) Oncogene 22: 484–97
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Immunogen
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p70 S6 Kinase (Ab-424) antibody was raised against a peptide sequence around aa.422~426 (P-V-S-P-V) derived from Human p70S6k.
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p70 S6 Kinase AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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IFIHCWB
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Species Reactivity
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Human
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Concentration
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1mg/ml
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Purification
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Affinity purified
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p70 S6 Kinase AntibodyTarget Information
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Gene Symbol
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RPS6KB1
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Alternative Names
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S6K
PS6K
S6K1
STK14A
p70-S6K
p70S6KA
p70-alpha
S6K-beta-1
p70(S6K)-alpha
PKR
PRKR
KS6B1
P70-S6K
RPS6KB1
S6K
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Molecular Weight(MW)
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70, 85 kDa
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Tissue Specificity
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This antibody detects endogenous level of total p70 S6 Kinase protein.
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p70 S6 Kinase AntibodyDatabase Links
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Entrez Gene
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6198
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Protein Accession
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NP_003152.1
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p70 S6 Kinase AntibodyApplication
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Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Ab-424). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using p70 S6 Kinase (Ab-424).
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Application
Western blot analysis of lysed extracts from 293 cells using p70 S6 Kinase (Ab-424).
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Application Notes
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store antibody at -20?C for up to one year.
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Storage Buffer
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Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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