如果您對該產(chǎn)品感興趣的話,可以
產(chǎn)品名稱: Mouse Raf1 Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
Mouse Raf1 Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。Mouse Raf1 Antibody嚴(yán)格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
Mouse Raf1 Antibody
的詳細(xì)介紹
Mouse Raf1 AntibodyProduct Profile
|
Product Name
|
Mouse Raf1 Antibody
|
|
Antibody Type
|
Primary Antibodies
|
|
Product description
|
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3 (By similarity).1) Niault, T.S., et al. Carcinogenesis 31(7):1165-1174(2010)
|
|
Immunogen
|
This Mouse Raf1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of mouse Raf1.
|
Mouse Raf1 AntibodyKey Feature
|
Clonality
|
Polyclonal
|
|
Isotype
|
Ig
|
|
Host Species
|
Rabbit
|
|
Tested Applications
|
FACSIHC-PWB
|
|
|
For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50:
|
|
Species Reactivity
|
HumanMouse
|
|
Concentration
|
1mg/ml
|
|
Purification
|
Affinity purified
|
Mouse Raf1 AntibodyTarget Information
|
Gene Symbol
|
Raf1
|
|
Alternative Names
|
RAF proto-oncogene serine/threonine-protein kinase
Proto-oncogene c-RAF
cRaf
Raf-1
Raf1
Craf
More
|
|
Molecular Weight(MW)
|
73 kDa
|
|
Sequence Similarities
|
Predicted species reactivity based on immunogen sequence: Rat
|
Mouse Raf1 AntibodyDatabase Links
|
Entrez Gene
|
110157
|
|
Protein Accession
|
Q99N57
|
Mouse Raf1 AntibodyApplication
-
Application
Mouse Raf1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
-
Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
-
Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
-
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).
|
Application Notes
|
For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50:
|
Additional Information
|
Form
|
Liquid
|
|
Storage Instructions
|
Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
|
|
Storage Buffer
|
Supplied in PBS with 0.09% (W/V) sodium azide.
|
