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產(chǎn)品資料

CYK18 Antibody

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產(chǎn)品名稱: CYK18 Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

CYK18 Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。CYK18 Antibody嚴格的質(zhì)量控制和驗證實驗包括各種不同的應用:WB、細胞**熒光、**組化、流式細胞術(shù)、ELISA、CHIP、IP和peptide array。


CYK18 Antibody  的詳細介紹


CYK18 AntibodyCYK18 AntibodyProduct Profile

Product Name CYK18 Antibody
Antibody Type Primary Antibodies
Product description
				
KRT18 is the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament family. They are expressed in single layer epithelial tissues of the body. Mutations in its gene have been linked to cryptogenic cirrhosis.1) Zhang,Q., Clin. Cancer Res. 15 (10), 3557-3567 (2009)
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Immunogen
This CYK18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from the C-terminal region of human CYK18.

CYK18 AntibodyKey Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

FACSIFIHC-PWB

For IF starting dilution is: 1:25 For FACS starting dilution is: 1:25:
Species Reactivity

HumanMouse

Concentration 1mg/ml
Purification Unpurified

CYK18 AntibodyTarget Information

Gene Symbol KRT18
Alternative Names
Keratin
type I cytoskeletal 18
Cell proliferation-inducing gene 46 protein
Cytokeratin-18
CK-18
Keratin-18
K18
KRT18
CYK18
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Molecular Weight(MW) 48 kDa

CYK18 AntibodyDatabase Links

Entrez Gene 3875
Protein Accession P05783

CYK18 AntibodyApplication

  • Application

    Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit Ig

  • Application

    Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit Ig

  • Application

    Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit Ig

  • Application

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoskeleton staining on HeLa cell line. The nuclear c

  • Application

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoskeleton staining on HeLa cell line. The nuclear c

  • Application

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoskeleton staining on HeLa cell line. The nuclear c

  • Application

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoskeleton staining on HeLa cell line. The nuclear c

Application Notes
For IF starting dilution is: 1:25 For FACS starting dilution is: 1:25:

Additional Information

Form Liquid
Storage Instructions Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.


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