p38 MAPK (phospho Thr180 / Tyr182) Antibody
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產(chǎn)品名稱: p38 MAPK (phospho Thr180 / Tyr182) Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
p38 MAPK (phospho Thr180 / Tyr182) Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。p38 MAPK (phospho Thr180 / Tyr182) Antibody嚴格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細胞**熒光、**組化、流式細胞術(shù)、ELISA、CHIP、IP和peptide array。
p38 MAPK (phospho Thr180 / Tyr182) Antibody
的詳細介紹
p38 MAPK (phospho Thr180 / Tyr182) AntibodyProduct Profile
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Product Name
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p38 MAPK (phospho Thr180 / Tyr182) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one these kinases and it is activated by both inflammatory cytokines and by stress. The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system. Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr180 and Tyr182.1) Shi, Y. and Gaestel, M. "In the cellular garden of forking paths: How p38 MAPKs signal for downstream assistance," Biol. Chem. 383 (2002) 1519 - 1536.
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Immunogen
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p38 MAPK (Thr180/Tyr182) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr180/Tyr182 of p38.
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p38 MAPK (phospho Thr180 / Tyr182) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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WB
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Immunolabeling in Western blots of C-6 glioma cells prepared with anisomycin treatment is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Immunoblotting (DB) and Western Blots (WB). Immunohistochemistry (IHC): not yet determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.:
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Species Reactivity
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HumanMouseRat
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Concentration
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1mg/ml
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Purification
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Affinity purified
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p38 MAPK (phospho Thr180 / Tyr182) AntibodyTarget Information
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Gene Symbol
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Mapk14
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Alternative Names
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RK
Hog
p38
CRK1
CSBP
Exip
Mxi2
CSPB1
Csbp1
Csbp2
Prkm14
Prkm15
Sapk2A
p38Hog
p38alpha
MAP kinase 14
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Molecular Weight(MW)
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39
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Tissue Specificity
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p38 MAPK antibody is specific for the ~39k p38 protein phosphorylated at Thr180/Tyr182.
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p38 MAPK (phospho Thr180 / Tyr182) AntibodyDatabase Links
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Entrez Gene
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81649
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Protein Accession
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P70618
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p38 MAPK (phospho Thr180 / Tyr182) AntibodyApplication
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Application
Western blot of Hela cell lysates that had been treated with +/- UV(30 min.) showing phosphospecific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180 and Tyr182.
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Application Notes
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Immunolabeling in Western blots of C-6 glioma cells prepared with anisomycin treatment is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Immunoblotting (DB) and Western Blots (WB). Immunohistochemistry (IHC): not yet determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.:
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Additional Information
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Form
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Liquid
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Storage Instructions
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For long term storage –80?C is recommended, but shorter term storage at –20?C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
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Storage Buffer
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100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
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