STAT1 (phospho Tyr701) Antibody
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產(chǎn)品名稱: STAT1 (phospho Tyr701) Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
STAT1 (phospho Tyr701) Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。STAT1 (phospho Tyr701) Antibody嚴(yán)格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
STAT1 (phospho Tyr701) Antibody
的詳細(xì)介紹
STAT1 (phospho Tyr701) AntibodyProduct Profile
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Product Name
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STAT1 (phospho Tyr701) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-γ-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.1) Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120. Durbin J E, et al. (1996) Cell. 84: 443-450. Meraz M A, et al. (1996) Cell. 84: 431-442. Wakao H, et al. (1994) EMBO J. 13: 2182-2191. Demoulin J, B. et al. (1999) J Biol Chem. 274: 25855-258 Ihle J N, et al. (1994) Trends Biochem Sci. 19: 222-227.
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Immunogen
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STAT1 (Phospho-Tyr701) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 701 (T-G-Y (p) -I-K) derived from Human STAT1.
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STAT1 (phospho Tyr701) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Species Reactivity
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Human
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Concentration
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1mg/ml
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Purification
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Affinity purified
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STAT1 (phospho Tyr701) AntibodyTarget Information
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Gene Symbol
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STAT1
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Alternative Names
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CANDF7
ISGF-3
STAT91
BHLHE39
Transcription factor ISGF-3 components p91/p84
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Molecular Weight(MW)
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91 kDa
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Tissue Specificity
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This antibody detects endogenous level of STAT1 only when phosphorylated at tyrosine 701.
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STAT1 (phospho Tyr701) AntibodyDatabase Links
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Entrez Gene
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6772
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Protein Accession
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NP_009330.1
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STAT1 (phospho Tyr701) AntibodyApplication
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Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT1 (Phospho-Tyr701).
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Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with IFN using STAT1 (Phospho-Tyr701).
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Application Notes
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store antibody at -20?C for up to one year.
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Storage Buffer
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Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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