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產(chǎn)品名稱: SMAD3-S208 Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
SMAD3-S208 Antibody迅速增長(zhǎng)的產(chǎn)品線包括一抗、二抗、**測(cè)定及細(xì)胞檢測(cè)試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。SMAD3-S208 Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
SMAD3-S208 Antibody
的詳細(xì)介紹
SMAD3-S208 AntibodyProduct Profile
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Product Name
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SMAD3-S208 Antibody
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Antibody Type
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Primary Antibodies
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Product description
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SMAD3 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.1) Zhang, M., et al. J. Biol. Chem. 285(12):8703-8710(2010)
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Immunogen
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This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from human SMAD3.
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SMAD3-S208 AntibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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Ig
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Host Species
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Rabbit
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Tested Applications
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FACSIFIHC-PWB
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For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:
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Species Reactivity
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HumanMouse
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Concentration
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1mg/ml
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Purification
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Affinity purified
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SMAD3-S208 AntibodyTarget Information
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Gene Symbol
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SMAD3
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Alternative Names
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Mothers against decapentaplegic homolog 3
MAD homolog 3
Mad3
Mothers against DPP homolog 3
hMAD-3
JV15-2
SMAD family member 3
SMAD 3
Smad3
hSMAD3
SMAD3
MADH3
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Molecular Weight(MW)
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48 kDa
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Sequence Similarities
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Predicted species reactivity based on immunogen sequence: Rat
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SMAD3-S208 AntibodyDatabase Links
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Entrez Gene
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4088
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Protein Accession
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P84022
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SMAD3-S208 AntibodyApplication
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Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Application
Confocal immunofluorescent analysis of SMAD3-S208 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
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Application
SMAD3-S208 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Application
Western blot analysis in HepG2 and mouse C2C12 cell line lysates (35ug/lane).
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Application Notes
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For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Storage Buffer
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Supplied in PBS with 0.09% (W/V) sodium azide.
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