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產(chǎn)品資料

SMAD3 Antibody

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產(chǎn)品名稱: SMAD3 Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

SMAD3 Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。SMAD3 Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。


SMAD3 Antibody  的詳細(xì)介紹

SMAD3 AntibodyProduct Profile

Product Name SMAD3 Antibody
Antibody Type Primary Antibodies
Product description 
					

						The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.1) Ge, Q., et al. J. Cell. Physiol. 225(3):846-854(2010)
					

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Immunogen 
This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-156 amino acids from the Central region of human SMAD3.

SMAD3 AntibodyKey Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

IFIHC-PWB

For WB starting dilution is: 1:1000 For IF starting dilution is: 1:100~500 For IHC-P starting dilution is: 1:50~200:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

SMAD3 AntibodyTarget Information

Gene Symbol SMAD3
Alternative Names
Mothers against decapentaplegic homolog 3
MAD homolog 3
Mad3
Mothers against DPP homolog 3
hMAD-3
JV15-2
SMAD family member 3
SMAD 3
Smad3
hSMAD3
SMAD3
MADH3
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Molecular Weight(MW) 48 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Chicken

SMAD3 AntibodyDatabase Links

Entrez Gene 4088
Protein Accession P84022

SMAD3 AntibodyApplication

  • Application

    SMAD3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Application

    Western blot analysis in CEM cell line lysates (35ug/lane).

  • Application

    Confocal immunofluorescent analysis of SMAD3 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Application Notes
For WB starting dilution is: 1:1000 For IF starting dilution is: 1:100~500 For IHC-P starting dilution is: 1:50~200:

Additional Information

Form Liquid
Storage Instructions Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.

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