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產(chǎn)品名稱: Mouse Mapk3 Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
Mouse Mapk3 Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。Mouse Mapk3 Antibody嚴(yán)格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
Mouse Mapk3 Antibody
的詳細(xì)介紹
Mouse Mapk3 AntibodyProduct Profile
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Product Name
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Mouse Mapk3 Antibody
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Antibody Type
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Primary Antibodies
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Product description
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Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2) and heat shock factor protein 4 (HSF4) (By similarity). Phosphorylates SPZ1.1) Chen, W., et al. Biochem. Biophys. Res. Commun. 401(3):339-343(2010)
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Immunogen
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This Mouse Mapk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-380 amino acids from the C-terminal region of mouse Mapk3.
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Mouse Mapk3 AntibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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Ig
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Host Species
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Rabbit
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Tested Applications
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FACSIHC-PWB
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For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
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Species Reactivity
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Mouse
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Concentration
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1mg/ml
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Purification
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Affinity purified
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Mouse Mapk3 AntibodyTarget Information
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Gene Symbol
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Mapk3
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Alternative Names
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Mitogen-activated protein kinase 3
MAP kinase 3
MAPK 3
ERT2
Extracellular signal-regulated kinase 1
ERK-1
Insulin-stimulated MAP2 kinase
MAP kinase isoform p44
p44-MAPK
MNK1
Microtubule-associated protein 2 kinase
p44-ERK1
Mapk3
Erk1
Prkm3
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Molecular Weight(MW)
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43 kDa
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Mouse Mapk3 AntibodyDatabase Links
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Entrez Gene
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26417
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Protein Accession
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Q63844
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Mouse Mapk3 AntibodyApplication
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Application
Mouse Mapk3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Application
Western blot analysis of lysates from mouse BA/F3, C2C12, NIH/3T3 and rat C6 cell line (from left to right), using Mouse Mapk3 Antibody at 1:1000 at each lane.
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Application
Flow cytometric analysis of Hela cells using Mouse Mapk3 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Application Notes
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For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Storage Buffer
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Supplied in PBS with 0.09% (W/V) sodium azide.
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