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產(chǎn)品名稱: TrkA-pY791 Antibody
產(chǎn)品型號:
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
TrkA-pY791 Antibody迅速增長的產(chǎn)品線包括一抗、二抗、**測定及細(xì)胞檢測試劑盒、蛋白/多肽、激動劑/拮抗劑/活化劑/抑制劑及裂解物等。TrkA-pY791 Antibody嚴(yán)格的質(zhì)量控制和驗證實驗包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
TrkA-pY791 Antibody
的詳細(xì)介紹
TrkA-pY791 AntibodyProduct Profile
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Product Name
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TrkA-pY791 Antibody
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Antibody Type
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Primary Antibodies
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Product description
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TRKA (also known as NTRK1)is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in the TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer.1) Tokusashi, Y., et al., Int. J. Cancer 114(1):39-45 (2005).
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Immunogen
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This TrkA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 769-796 amino acids from human TrkA.
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TrkA-pY791 AntibodyKey Feature
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Clonality
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Polyclonal
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Isotype
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Ig
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Host Species
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Rabbit
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Tested Applications
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FACSIHC-PWB
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For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000:
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Species Reactivity
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HumanMouse
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Concentration
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1mg/ml
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Purification
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Affinity purified
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TrkA-pY791 AntibodyTarget Information
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Gene Symbol
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NTRK1
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Alternative Names
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High affinity nerve growth factor receptor
Neurotrophic tyrosine kinase receptor type 1
TRK1-transforming tyrosine kinase protein
Tropomyosin-related kinase A
Tyrosine kinase receptor
Tyrosine kinase receptor A
Trk-A
gp140trk
p140-TrkA
NTRK1
MTC
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Molecular Weight(MW)
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87 kDa
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TrkA-pY791 AntibodyDatabase Links
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Entrez Gene
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4914
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Protein Accession
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P04629
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TrkA-pY791 AntibodyApplication
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Application
Overlay histogram showing SH-SY5Y cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(O
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Application
Antibody staining TrkA in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Application
Western Blot at 1:2000 dilution + mouse brain lysate Lysates/proteins at 20 ug per lane.
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Application
Western blot analysis of hTrkA-pY791 in HepG2 cell line lysates (35ug/lane)
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Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Application
TrkA-pY791 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Application Notes
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For FACS starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store at 4?C for three months and -20?C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Storage Buffer
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Supplied in PBS with 0.09% (W/V) sodium azide.
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