Src (phospho Tyr529) Antibody
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產(chǎn)品名稱: Src (phospho Tyr529) Antibody
產(chǎn)品型號(hào):
產(chǎn)品展商: immunoclone
產(chǎn)品文檔: 無(wú)相關(guān)文檔
簡(jiǎn)單介紹
Src (phospho Tyr529) Antibody迅速增長(zhǎng)的產(chǎn)品線包括一抗、二抗、**測(cè)定及細(xì)胞檢測(cè)試劑盒、蛋白/多肽、激動(dòng)劑/拮抗劑/活化劑/抑制劑及裂解物等。Src (phospho Tyr529) Antibody嚴(yán)格的質(zhì)量控制和驗(yàn)證實(shí)驗(yàn)包括各種不同的應(yīng)用：WB、細(xì)胞**熒光、**組化、流式細(xì)胞術(shù)、ELISA、CHIP、IP和peptide array。
Src (phospho Tyr529) Antibody
的詳細(xì)介紹
Src (phospho Tyr529) AntibodyProduct Profile
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Product Name
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Src (phospho Tyr529) Antibody
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Antibody Type
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Primary Antibodies
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Product description
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proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.1) Pyper J. M., (1985) Mol. Cell. Biol. 5:831-838Pyper J. M. (1990) Mol. Cell. Biol. 10:2035-2040Xu W., (1997) . Nature 385:595-602Benes C. H., (2005) Cell 121:271-280
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Immunogen
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Src (Phospho-Tyr529) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y (p) -Q-P) derived from Human Src.
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Src (phospho Tyr529) AntibodyKey Feature
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Clonality
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Polyclonal
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Host Species
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Rabbit
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Tested Applications
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IFIHCWB
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Species Reactivity
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HumanMouseRat
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Concentration
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1mg/ml
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Purification
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Affinity purified
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Src (phospho Tyr529) AntibodyTarget Information
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Gene Symbol
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SRC
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Alternative Names
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ASV
SRC1
c-SRC
p60-Src
PKB
RAC
C-SRC
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Molecular Weight(MW)
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60 kDa
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Tissue Specificity
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This antibody detects endogenous level of Src only when phosphorylated at tyrosine 529.
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Src (phospho Tyr529) AntibodyDatabase Links
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Entrez Gene
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6714
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Protein Accession
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NP_005408.1
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Src (phospho Tyr529) AntibodyApplication
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Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (Phospho-Tyr529). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Src (Phospho-Tyr529).
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Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using Src (Phospho-Tyr529).
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Application Notes
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Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
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Additional Information
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Form
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Liquid
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Storage Instructions
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Store antibody at -20?C for up to one year.
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Storage Buffer
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Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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