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NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF. NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia.1) Vascotto,C., Mol. Cell. Biol. 29 (7), 1834-1854 (2009) Ma,W., Cancer Biomark(1), 51-58 (2009) Zhang,H., J. Biol. Chem. 279 (34), 35726-35734 (2004) More
This NPM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-226 amino acids from the C-terminal region of human NPM1.
FACSIFIHC-PWB
Human
NPM1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Hela,Jurkat cell line lysates (35ug/lane).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Fluorescent image of A549 cell stained with NPM1 Antibody . A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPM1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/
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