beta-Actin (Loading Control)抗體做wb時，一抗和二抗?jié)舛仁窃趺创_定的？怎么設(shè)置抗體滴度：
一抗的用量和樣品中的目標分子含量相關(guān)，如果含量較高，就不需要用太多一抗，以免浪費和條帶太亮不易分析。推薦采用廠家的抗體濃度（義翹神州會提供抗體用量、天然細胞株檢測結(jié)果），如果樣品與廠家的不是一種細胞，可以嘗試上下摸索一兩個濃度梯度；
二抗的用量，和你所在實驗室常規(guī)的用量就可以，如果出現(xiàn)效果不理想的狀態(tài)，二抗的作用可以通過陽性對照和陰性對照、以及其他人的使用效果看出來有沒有問題。
英文名稱：beta-Actin (Loading Control)抗體
中文名稱β-肌動蛋白/β-Actin 抗體（內(nèi)參抗體）
別 名Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin;A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN.
JournalPMIDIFApplicationBiomaterials (2013)238100817.6040WBBiomaterials (2013)237779167.6040WBNanotoxicology (2015)263995856.4110WBArteriosclerosis, thrombosis, and vascular biology (2008)185997966.3380WBArchives of toxicology (2012)224223405.2150WBMolecular Neurobiology (2015)263855765.1370WBMolecular Cancer (2013)242156325.1300WBBritish Journal of Pharmacology (2014)248353595.0670WBEndocrinology (2015)262874024.5030WBMolecular Pharmacology (2013)234702874.4110WBCancer letters (2007)182268554.2580WBBiochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids (2010)203822594.1340 WB Nanotechnology (2013) 23425941 3.8420 WB Nanotechnology (2013) 23425941 3.8420 WB Proteomics (2016) 27173832 3.8100 WB The Journal of Nutritional Biochemistry (2014) 24794015 3.7940 WB PloS One (2012) 23028660 3.7300 WB PloS One (2013) 23922700 3.7300 WB PloS One (2013) 24098763 3.7300 WB Cancer Immunology, Immunotherapy (2013) 24077852 3.6370 WB Colloids and Surfaces B: Biointerfaces (2012) 22503631 3.5540 WB PLoS One (2015) 26266800 3.5340 WB Cellular Physiology and Biochemistry (2014) 24556756 3.4150 WB Free radical research (2007) 17907002 3.2790 WB Developmental & Comparative Immunology (2012) 23178402 3.2380 WB/IP Developmental & Comparative Immunology (2013) 23545285 3.2380 WB Developmental & Comparative Immunology (2013) 23500512 3.2380 WB Developmental & Comparative Immunology (2013) 23932982 3.2380 WB Developmental & Comparative Immunology (2012) 23178402 3.2380 WB International Journal of Biological Sciences (2014) 24719562 3.1680 WB Toxicology Letters (2011) 21232584 3.1450 WB Neuroscience (2011) 21219973 3.1220 WB Diabetes/Metabolism Research and Reviews (2012) 21928330 2.9680 WB Chemico-Biological Interactions (2012) 24025711 2.9670 WB Fish & Shellfish Immunology (2012) 23063540 2.9640 WB Journal of Cancer Research and Clinical Oncology (2012) 22806311 2.9140 WB Food and Chemical Toxicology (2016) 27237588 2.9000 WB Brain Research Bulletin (2016) 26876758 2.7180 WB Life Sciences (2016) 26924495 2.7020 WB Nutrition and Cancer (2014) 24660968 2.6950 WB Neurochemistry international (2013) 24220540 2.6590 WB Neurochemistry international (2013) 24274996 2.6590 WB Neurochemistry international (2011) 21185898 2.6590 WB Life Science Journal (2013) Others 2.5550 WB Toxicology and Applied Pharmacology (2016) 27105818 2.5320 WB Tumor Biology (2013) 23539435 2.5180 WB Acta Pharmacologica Sinica (2015) 25937633 2.4960 WB World Journal of Gastroenterology (2015) 25663771 2.4330 WB Biochemical and Biophysical Research Communications (2013) 24211202 2.4060 WB Acta Pharmacologica Sinica (2014) 24632846 2.3540 WB Acta Pharmacologica Sinica (2014) 24909515 2.3540 WB Molecular and Cellular Biochemistry (2011) 21553022 2.3290 WB Molecular and Cellular Biochemistry (2014) 24833465 2.3290 WB Rheumatology international (2010) 19847436 2.2140 WB Medical Oncology (2013) 23536000 2.1470 WB Reproductive Biology and Endocrinology (2013) 23452984 2.1400 WB Neurochemical Research (2013) 23780673 2.1250 WB Virology Journal (2011) 21392403 2.0900 WB Respiratory physiology & neurobiology (2013) 23665052 2.0510 WB International Journal of Clinical and Experimental Pathology (2015) 26261494 1.8910 WB Research in Veterinary Science (2012) 23164636 1.7740 WB Cellular Immunology (2013) 23774531 1.7430 WB Applied Biochemistry and Biotechnology (2016) 27193254 1.7400 WB Poultry Science (2014) 24570433 1.7280 WB Nephrology (2011) 21771176 1.6880 WB Biological Trace Element Research (2015) 25876085 1.6080 WB Biological trace element research (2013) 23575899 1.3070 WB Others 1.2770 WB Brazilian Journal of Medical and Biological Research (2014) 25517918 1.1390 WB Experimental & Clinical Cardiology (2013) 23940455 1.1000 WB World Journal of Surgical Oncology (2014) 24885858 1.0900 WB CHINESE MEDICAL JOURNAL-BEIJING-ENGLISH EDITION (2009) 19236815 0.9010 WB Pediatrics International (2016) 27097979 0.7300 WB Experimental and Therapeutic Medicine (2014) 24396436 0.3440 WB
說 明 書50ul 100ul 200ul
產(chǎn)品類型內(nèi)參抗體
研究領(lǐng)域腫瘤 細胞生物 信號轉(zhuǎn)導(dǎo) 細胞骨架
抗體來源Rabbit
克隆類型Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Bee, Fish, Guinea Pig, Hamster, Goat, Cat, mt,op
產(chǎn)品應(yīng)用WB=1:5000-20000 ELISA=1:5000-20000 IHC-P=1:500-1000 IHC-F=1:500-1000 Flow-Cyt=1μg/Test IF=1:100-1000 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
beta-Actin (Loading Control)抗體分 子 量42kDa
細胞定位細胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原Synthetic MAP peptide derived from human beta-Actin
亞 型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C
beta-Actin (Loading Control)抗體ELISA如何選一抗與二抗：
一抗是與底物相結(jié)合的，同時一抗必須有抗原識別位點，能被另一種抗體識別并結(jié)合。
二抗是能與一抗的抗原識別位點識別并結(jié)合，同時，二抗必須有可以觀測、計量的性質(zhì)。
這些性質(zhì)可以是：
①二抗本身帶有顏色，可以通過分光度儀檢測到二抗的濃度；
②二抗還可以作為一種酶，催化另一種底物，通過檢測底物或者生成物的濃度檢測出二抗的濃度；等等……有很多，就是說必須是可測量的。
beta-Actin (Loading Control)抗體保存得當與否，直接決定了抗體的活性和使用效果！如果抗體保存得當，大部分抗體活性都可以維持數(shù)月甚至數(shù)年。收到抗體后請務(wù)必在12000rpm離心20-30s后再打開管蓋進行分裝和保存！抗體使用非常特殊的儲存管，只有在12000rpm離心20-30s才能將附著在管壁或蓋內(nèi)的抗體全部離心下來。即使是在10000rpm離心也會導(dǎo)致離心不完全，導(dǎo)致出現(xiàn)抗體的量不足的現(xiàn)象！
