Histone H3.1抗體可供應(yīng)相應(yīng)抗體的生物素、辣根過(guò)氧化物酶、熒光素（FITC、PE、  CY3、 CY5、 CY7、 PE- CY3/5/7)標(biāo)記抗體，我司主推四大類：一. 抗體（antibody）：182000種的一抗?？蓱?yīng)用于Western blot，IP，IHC，IF，F(xiàn)ACS，ELISA等實(shí)驗(yàn)。二. 蛋白(protien)：20000種的高純度蛋白。三. elisa試劑盒(elisa kit)：可以提供超過(guò)6600種的ELISA試劑盒，包括人源，大鼠源，小鼠源等。四. 小分子(Small Molecules)：超過(guò)25000種的生物活性小分子，主要包括：拮抗劑、抑制劑、***、天然產(chǎn)物。

英文名稱：Histone H3.1抗體

中文名稱組蛋白H3.1抗體

別    名H3 histone family, member A; H3 histone family, member B; H3 histone family, member C; H3 histone family, member D; H3 histone family, member E; H3 histone family, member F; H3 histone family, member G; H3 histone family, member H; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3.1; H3.f; H3/A; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H31_HUMAN; H3F1K; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1 cluster H3A; HIST1 cluster H3B; HIST1 cluster H3C; HIST1 cluster H3D; HIST1 cluster H3E; HIST1 cluster H3F; HIST1 cluster H3G; HIST1 cluster H3I; HIST1 cluster H3J; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; histone 1, H3b; Histone 1, H3c; Histone 1, H3e; histone 1, H3f; histone 1, H3g; histone 1, H3h; histone 1, H3i; histone 1, H3j; histone cluster 1, H3a; histone cluster 1, H3b; Histone cluster 1, H3c; Histone cluster 1, H3e; histone cluster 1, H3g; histone cluster 1, H3h; histone cluster 1, H3i; histone cluster 1, H3j; Histone gene cluster 1 H3A; Histone gene cluster 1 H3B; Histone gene cluster 1 H3E; Histone gene cluster 1 H3F; Histone gene cluster 1 H3G; Histone gene cluster 1, H3; Histone gene cluster 1, H3C; Histone gene cluster 1, H3D; Histone gene cluster 1, H3I; Histone gene cluster 1, H3IH; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l  

說(shuō) 明 書100ul  200ul

研究領(lǐng)域細(xì)胞生物  表觀遺傳學(xué)  

抗體來(lái)源Rabbit

克隆類型Polyclonal

交叉反應(yīng) Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, Chimpanzee,

產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量15kDa

細(xì)胞定位細(xì)胞核

性    狀Lyophilized or Liquid

濃    度1mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from human Histone H3.1

亞    型IgG

純化方法affinity purified by Protein A

儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Histone H3.1抗體特異性問(wèn)題。從本質(zhì)上來(lái)說(shuō)，抗體的工作原理就是與特定蛋白或其他抗原結(jié)合，從而實(shí)現(xiàn)定性，定量，定位等檢測(cè)。所以，抗體能否**的結(jié)合抗原，是成敗的關(guān)鍵，抗體的特異性（specificity)就顯得尤其重要了。而對(duì)特異性影響*大的，莫過(guò)于抗體的交叉反應(yīng)性(cross-reactivity)。在特異性正常情況下，Histone H3.1抗體只能結(jié)合**的一種蛋白。而如果抗體本身出現(xiàn)問(wèn)題（如結(jié)構(gòu)等），可能會(huì)結(jié)合其它非目標(biāo)蛋白，從而導(dǎo)致特異性降低。更令人惱火的是，如果抗體的特異性問(wèn)題很難被檢測(cè)出來(lái)。假設(shè)某個(gè)糟糕的抗體X理論上只結(jié)合蛋白A，但是它也可以結(jié)合蛋白B。當(dāng)實(shí)驗(yàn)樣本里蛋白B沒(méi)有出現(xiàn)時(shí)，它將完全得到完全正確的實(shí)驗(yàn)結(jié)果。如果當(dāng)樣本中包含蛋白B，偏差就出現(xiàn)了。試想一下，你在應(yīng)用抗體X時(shí)，如果你在項(xiàng)目前期沒(méi)有使用過(guò)蛋白B樣本，一切都會(huì)很順利。但是在項(xiàng)目后期，你開(kāi)始加入蛋白B樣本，你的實(shí)驗(yàn)結(jié)果就完全亂了套。那么問(wèn)題就來(lái)了：你事先并不知道抗體X可以結(jié)合非目標(biāo)蛋白B，所以你完全無(wú)法解釋為什么項(xiàng)目的前后會(huì)出現(xiàn)偏差。

Histone H3.1抗體出現(xiàn)交叉反應(yīng)，導(dǎo)致特異性降低，主要是由抗體本身的質(zhì)量問(wèn)題引起的。由于抗體本身結(jié)構(gòu)和生產(chǎn)過(guò)程的復(fù)雜性，生產(chǎn)商稍有疏忽，抗體質(zhì)量就會(huì)出現(xiàn)問(wèn)題。由于抗體是科研的剛需產(chǎn)品，利潤(rùn)又很高，所以引得眾多公司瘋狂進(jìn)入到這個(gè)市場(chǎng)，直接導(dǎo)致了市面抗體產(chǎn)品質(zhì)量良莠不齊，供應(yīng)商魚龍混雜。而市場(chǎng)本身又缺少有力的監(jiān)督和管理機(jī)制，抗體頻頻出現(xiàn)質(zhì)量問(wèn)題也就不足為奇了。