英文名稱:alpha smooth muscle Actin抗體
中文名稱肌動(dòng)蛋白α/α-SMA/α Actin抗體
別 名alpha sarcomeric Actin; alpha smooth muscle Actin; Actin alpha; ASMA; ASM-A; alpha-SMA; alpha SMA; AAT6; ACTA2; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; ACTSA; ACTVS; Alpha 2 actin; Alpha-actin 2; Cell growth inhibiting gene 46 protein; Growth inhibiting gene 46; ACTA_HUMAN; Actin alpha 2 smooth muscle aorta; Actin aortic smooth muscle; Actin, aortic smooth muscle; Alpha 2 actin; Alpha actin 2; Alpha cardiac actin; Alpha-actin 2; Alpha-actin-2; Cell growth inhibiting gene 46 protein; Cell growth-inhibiting gene 46 protein; Growth inhibiting gene 46; MYMY5 α-Smooth Muscle Actin; α Smooth Muscle Actin;
JournalPMIDIFApplicationPLoS One (2014)249191893.7300WBAmerican Journal of Physiology-Renal Physiology (2011)217956413.6120IHC-PMicrovascular Research (2013)239783342.9290IFWorld journal of urology (2009)188463792.8880noBrain Research (2013)235420552.8790IHC-PActa Pharmacologica Sinica (2010)211131772.3540WBCardiovascular and interventional radiology (2013)236524182.1380IHC-PBMC Urology (2014)251488491.9400IHC-P
alpha smooth muscle Actin抗體說(shuō) 明 書50ul 100ul 200ul
研究領(lǐng)域腫瘤 細(xì)胞生物 **學(xué) 細(xì)胞骨架
抗體來(lái)源Rabbit
克隆類型Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Fish, Guinea Pig, Hamster, Cat, HMt,Op
產(chǎn)品應(yīng)用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復(fù))
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量42kDa
細(xì)胞定位細(xì)胞漿
性 狀Lyophilized or Liquid
濃 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Actin alpha C-terminus
亞 型IgG
純化方法affinity purified by Protein A
儲(chǔ) 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
alpha smooth muscle Actin抗體選擇指南:
檢測(cè)任何目的靶蛋白都有不止一種抗體可供選擇,為縮小抗體的選擇范圍選中合適的抗體,需要考慮如下幾種因素:
(1) 分析或應(yīng)用的類型
(2)樣本蛋白的結(jié)構(gòu)性質(zhì)
(3)樣本的種屬
(4)抗體宿主的種類
(5)抗體的標(biāo)記和檢測(cè)
1 alpha smooth muscle Actin抗體分析試驗(yàn)的應(yīng)用類型一般抗體說(shuō)明書都列出該抗體經(jīng)試驗(yàn)驗(yàn)證過(guò)適用于何種分析類型,如:
可以應(yīng)用于WB IHC ICC ELASA 分析等,如果抗體說(shuō)明書沒(méi)有提及的應(yīng)用類型,并不意味著該抗體不適用于此種分析應(yīng)用類型,而僅是說(shuō)明尚未經(jīng)過(guò)此種分析試驗(yàn)驗(yàn)證,如果抗體不適用某些分析試驗(yàn),則會(huì)在抗體說(shuō)明書上標(biāo)注出來(lái)不適于某分析試驗(yàn)。
2 樣本蛋白的結(jié)構(gòu)性質(zhì)了解樣本蛋白的結(jié)構(gòu)性質(zhì)有助于選擇*合適的抗體,至少需要考慮兩方面因素
(1)..待測(cè)樣本蛋白的結(jié)構(gòu)域:抗體是由各種不同**原**宿主而制備得來(lái),其中的**原包括:全長(zhǎng)蛋白、蛋白片斷、多肽、全有機(jī)體(如:**)或細(xì)胞,alpha smooth muscle Actin抗體說(shuō)明書一般都有**原的描述,如果打算檢測(cè)的是蛋白片斷或一種特殊的同型物或蛋白全長(zhǎng)的某一區(qū)域,則必須選擇用含此片段域的**原制備出的抗體。如果打算用FACS 流式檢測(cè)活細(xì)胞的表面蛋白,則需要選擇含該表面蛋白的胞外域來(lái)**制備的抗體。
二抗的選擇 二抗應(yīng)選用與使用的一抗相同的物種來(lái)源,例如:如果你的一抗是小鼠的單克隆抗體,二抗則選抗小鼠的二抗anti-mouse
secondary。建議檢查二抗說(shuō)明書確保該抗體適用于你的檢測(cè)應(yīng)用, 二抗一般連接熒光素FITC 或發(fā)光團(tuán)。